IMMUNE 3D ® presents several competitive and scientific advantages compared to other commonly used 3D platforms.
Advantages vs. Transwell Assays
- No artificial inserts compared to Transwell and Invasion assays
- Enables the growth and culture of tumor cells
- Chemokines are produced by the growing tumor cells in concentrations like those found in tumor tissues in vivo
- The culture system is three-dimensional and physiologically relevant
- Collagen and fibroblasts provide stromal components important for T cell migration and activation
- Termination of T cell migration over 4-9 days
- T cells migrate through a distance of 500µm
- Does not depend on the formation and uniformity issues of tumor spheroids
Advantages vs. Other 3D platforms
- Physiologically relevant platform designed to mimic the extracellular matrix
- Study migration and infiltration on real time, patient derived samples
- Multi-cells – immune-tumor interactions
- Not dependent on tumor spheroid formation or uniformity of spheroids across wells
- Time course imaging of immune cell migration
- Broad spectrum applications across R&D
Advantages vs. In vivo models
- Study effects of additional tumor immune cell infiltrates
- Enables cross reactivity to select human T-cell receptors
- Stringent environment to study antigen presentation under disease conditions
- Ability to study cell heterogeneity
- CTL lysis and peptide recognition compared to in vivo models
- Avoids TcR skewing when cells are removed
- Adequate T-cell homing in vivo