IMMUNE 3D ® is a unique and proprietary platform that is designed to capture the core cell types to effectively capture the extracellular matrix. The platform was initially designed to naturally observe the migration patterns of immune cells and to interrogate the stromal environments that cells need to permeate to reach their destinations. Over the last few years, we have applied our understanding of the role that immune cells play in various diseases into developing 3D models that capture the disease biology in an immune context.

Platform Capabilities

Lymphocyte Activation

The platform design enables the selective migration of T cells resulting in specific tumor lysis

Cross section imaging

Unique and patented design enables cross sectioning of the culture to observe and quantify the migration of T cells (and other immune cells) and apoptosis of tumors in response to drug treatment. This design lends itself to study the “hot” and “cold” tumor phenomenon in cancer immunotherapy.

T cell kinetics

Compared to other 3D platforms and pre-clinical systems, IMMUNE 3D is designed to monitor and measure the kinetics of T cells and lymphocytes.

Stromal Factors

The addition of human collagen and fibroblasts enables the study of the tumor stromal components involved in T cell migration.

Characterization of Tumor Infiltrating Lymphocytes (TILS)

The platform has been used to characterize patient derived TILs. This is highly valuable in isolating TCRs and characterizing specific immune cell populations of interest. Here we show an example of characterization of TILs for chemokines.

Tumor lysis imaging

Monitor the time course of tumor lysis in response to drug treatment over several days.

T Cell Receptor (TcR) Chains

Comparable TCRs chain preservation compared to in vivo makes it ideal for screening drug candidates in compliment to animal models

Comparison vs. 2D cultures

The acceptance of the tumor microenvironment (TME) playing a key role in cancer immunotherapy, IMMUNE 3D is well suited to capture the TME components. The inclusion of stromal components impacts cytokine and cell surface expression patterns.